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Fig. 6. Neurotrophin 4/5 (NT-4/5) mRNA in rat soleus becomes upregulated earlier during postnatal development than do the myosin heavy chain (MyHC) I/b (slow) and MyHC IIA (fast) mRNAs. PCR products of reverse-transcribed RNA extracted from soleus muscles (four at each age) using primer pairs for the 18S rRNA and competimers in conjunction with primer pairs for either (A) MyHC I/b, (B) MyHC IIA or (C) NT-4/5 at postnatal age 4 days (P4), 7 days (P7), 14 days (P14), 21 days (P21) and 28 days (P28). Arrows show the postnatal ages at which increases in MyHC I/b, MyHC IIA and NT-4/5 mRNA synthesis occur. Note that a different set of primer pairs for 18S rRNA and competimers (product size 489 bp) was used to visualize the MyHC IIA product (310 bp). nRT = no reverse transcription. (D) The relative levels of MyHC I/b, MyHC IIA and NT-4/5 mRNAs. Values represent means ± S.E.M. *=P<0.05 vs day 4; {dagger}=P<0.5 vs day 7.