Fig. 1. Morphologically discrete mitochondria in living cells. (A) Volume render of pancreatic acini loaded with the dye TMRE (0.5 µmol 1^-1, 20 min). The approximate locations of individual acinar cells are outlined in the inset image. Note that this image essentially shows the sub-plasmalemmal mitochondria around the perimeter of the individual acinar cells. (B–E) Surface render of mitochondrially targeted DsRed1-expressing cells. (B) PAE cells, (C) SH-SY5Y cells, (D) COS-7 cells and (E) cortical astrocytes. See text for details. Scale bars, 5 µm.