Fig. 4. LY294002 inhibits mitochondrial accumulation at sites of nerve growth
factor (NGF) stimulation. LY294002 treatment prevented the accumulation of
mitochondria in the region of NGF stimulation (N=12; paired
t-test). Dimethylsulfoxide (DMSO; vehicle) alone did not affect the
mitochondrial response to NGF stimulation. The density of mitochondria is
3-fold higher in the 10 µm region of the axon adjacent to the NGF-coated
bead than in similar proximal and distal non-bead control regions
(N=9; paired t-test). * represents statistical significance
at P
0.001.