Fig. 3. Nerve growth factor (NGF)-coated beads do not affect the cytoskeleton. Primary cultured DRG neurons were double immunostained for microtubules (MTs) and F-actin (MFs). In all panels, the positions of the NGF-coated beads prior to fixation are indicated by circles. The regions of the axons in contact with NGF-coated beads have MT arrays and MF density essentially identical to control regions of the axon not contacting a bead. Scale bar, 10 µm