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Fig. 9. Immunoblot analysis of actin associated with isolated chloroplasts from Spinacia oleracea. The association of actin with the isolated intact chloroplasts from spinach leaves was examined by immunoblotting of the chloroplasts immediately after isolation (lane 1), and incubation for 0 min (lane 2) and 30 min (lane 3) with exogenously added chicken skeletal muscle F-actin. The arrow indicates the position of the actin band. Molecular markers are indicated on the left.