Fig. 4. Western blot analysis of zebrafish tissues with antibodies specific for vacuolar ATPase subunit B isoforms. 30 µg of total protein were applied to each lane. Antibody #1034 recognizes both isoforms (B) whereas antibody #1035 (A) is specific for subunit isoform vatB1. Both antibodies revealed bands of approximately 55 kDa. SB, swimbladder tissue.