Fig. 3. Tissue distribution of vatB1 and vatB2. RT-PCR analysis of V-ATPase subunit B isoforms 1 and 2 in different tissues of D. rerio. 500 ng of total RNA were transcribed to cDNA using MLVV-reverse transcriptase and Random N6 Primers PCR was performed with isoform-specific primers. PCR-products were run on a 1% agarose gel and stained with ethidium bromide. Both isoforms are expressed in all tested tissues (A, vatB2 PCR product of 454 bp; B, vatB2 PCR products of 409 bp). SB, swim bladder; C, control (no template).