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Fig. 2. Determination of recombinant zebrafish (rZf) vatB1 and vatB2 by western blot analysis. Non-purified bacterially expressed zebrafish vacuolar ATPase subunit B isoforms vatB1 and vatB2 were probed with (A) antibody #1034, which recognizes both isoforms of the B subunit, and (B) antibody #1035, which is specific for vatB1.