Fig. 2. Synthesis of myofibrillar proteins in the wild-type (N2) and thick filament null mutants. Synchronized animals at larval stage 3 were pulse-labeled for 2 h with high specific activity ³⁵S-labeled bacteria and the relative synthetic rates of myosin and paramyosin determined by normalization to actin. As is evident in the autoradiogram (A), compared with N2 (lane a), there is a marked reduction in the rates of synthesis of myosin in both the myosin B null CB190 (lane b) and the paramyosin null CB1214 (lane c). The results of the densitometric analysis based on four independent determinations on each strain are presented in (B). Values are means ± S.E.M.