Fig. 2. Fluorescence micro-photographs of A. gambiae Sua 4.0 cells and A. stephensi larvae. In A–D, column 1 shows DsRed fluorescence, column 2 shows EGFP fluorescence and column 3 shows DsRed fields superimposed upon EGFP fields to show co-localisation (yellow). (A) Sua 4.0 cells transfected with pMinEGFP and pMiRED show perfect co-localisation. (B) Sua 4.0 cells transfected with pMinEGFP and pIR-EGFP₄₆₅-Linker show virtually no EGFP expression. (C) Wild-type A. stephensi larvae injected with pMinEGFP and pMiRED show exact co-localisation. (D) Wild-type A. stephensi larvae injected with pMinEGFP and pIR-EGFP₄₆₅-Linker show very limited EGFP expression. Cells were captured on a Nikon inverted microscope at 20x magnification 48 h post-transfection. Larvae were photographed at 10x magnification 48 h post-hatching. (E) Four-day-old EGFP-expressing A. stephensi larvae from transgenic line V_B injected with pMiRED (column 1) or pIR-EGFP₄₆₅-Linker (column 2). Column 3 shows localisation of DsRed expression from pIR-EGFP₄₆₅-Linker in the same larva.