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Fig. 1. Changes of relative cell water volume (Vr) of trout hepatocytes exposed to (A) hyposmotic medium or (B) hyperosmotic medium, followed by re-exposure to isosmotic conditions. C, A and B denote isosmotic control saline, hyposmotic calibration saline (264 mosmol l–1) and hyperosmotic calibration saline (308 mosmol l–1), respectively. HYPO EXP (166 mosmol l–1) and HYPER EXP (465 mosmol l–1) indicate duration of experimental hypo- and hyperosmotic exposure. Values are means ± S.E.M. of 60 cells from four independent preparations.