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Fig. 2. Western blots. (A) Distribution of caveolin in trout kidney (Kid), spleen (Spl), adipose (Adi) and liver (Liv). All lanes, 15 µg total protein. (B–E) Samples loaded: 30 µg crude homogenate (Hom) and 15 µg plasma membrane (PM), raft-depleted PM (RDPM) and raft-enriched PM (raft). Probed with (B) antibodies against ß2 adrenergic receptor (ß2AR), (C) adenylyl cyclase (AC), (D) insulin receptor ß subunit (IRß) and (E) clathrin (Clath).