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Fig. 3. (A) Near-ultraviolet circular dichroism (CD) spectra of Gillichthys mirabilis and G. seta muscle-type lactate dehydrogenases (A4-LDHs) at 20 °C, and the subtraction spectrum (G. mirabilis minus G. seta; MIR minus SETA). (B) Second-derivative absorbance spectra of the two forms at 20 °C and the subtraction spectrum. The peak-to-trough distances, a and b, used to calculate the ratio r are illustrated. Note the difference in x-axis range between the two panels. Mir, to be defined; SETA, to be defined; {Theta}MRW, residue ellipticity; d2A/dx2, second derivative of the spectrum.