Fig. 2. (A) Buffer-corrected far-ultraviolet circular dichroism (CD) spectra of Gillichthys mirabilis and G. seta muscle-type lactate dehydrogenases (A₄-LDHs) at 20 and 40 °C. (B) Thermal denaturation profiles of the A₄-LDH forms monitored using CD spectroscopy; absorbance was measured at 222 nm. Curves were fitted as described in the text; G. mirabilis A₄-LDH T_m=60.2±0.1 °C and G. seta A₄-LDH T_m=58.4±0.1 °C). MRW, residue ellipticity.