Fig. 3. Evaluation of kfCFTR immunofluorescent MR cells into those containing diffuse only, punctate only, or both diffuse and punctate distribution. Bars indicate the percentage of cells in each category; the numbers of cells measured are shown. The number of animals in each group were FW, 9; FW to SW for 24 h, 4; FW to SW for 48 h, 6; SW, 5. In FW animals, most cells had only diffuse kfCFTR immunofluorescence; 8.1 % (18 cells) had both. The percentage of cells with punctate distribution increased with acclimation to SW, and in fully adapted SW animals all cells had only apical punctate kfCFTR localization.