JEB -- Perzov et al. 205 (9): 1209 Figure IG7

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Fig. 7. Differential effect of stv1 ${Delta}$ and vph1 ${Delta}$ mutants on Pma1p sorting and CPY and ALP processing. (A) Sucrose density gradients of whole cell extracts from wild type (WT), stv1 ${Delta}$ , vph1 ${Delta}$ and stv1 ${Delta}$ /vph1 ${Delta}$ strains were prepared as described in Materials and methods. The location of Pma1p was determined by immunoblot analysis with polyclonal antibody against purified Pma1p. Fraction numbers are indicated at the bottom. (B) Western analysis of vacuoles isolated from wild type, stv1 ${Delta}$ and vph1 ${Delta}$ strains by Ficoll gradient centrifugation and adjusted to a protein concentration of 2 mg ml^-1 with antibodies against CPY and ALP. p, precursor of ALP; p1 and p2, precursors of CPY; m, mature form of the protein.