
Fig. 6. stv1
and vph1
strains show defects in
vacuolar acidification. Wild-type (WT) and V-ATPase mutants were grown in YPD
medium, pH 5.5, and stained with LysoSensor Green DND-189 as described in
Materials and methods. Staining of vph1
and
stv1
/vph1
strains is shown at two different light
intensities (*3 is triple intensity). The panel below shows
staining of vph1
grown in YPD buffered at pH 7.5.