Fig. 1. Null mutation of VPH1 causes overexpression of Stv1p. (A) Western analysis of whole cell extracts of wild type (WT) and stv1 mutants. Stv1p is absent from the stv1 strain. Some non-specific bands are labeled by the polyclonal antibody. The positions of marker proteins (kDa) are shown. (B) Western analysis of wild type, vph1, stv1 and vma8 strains. Whole cell extracts from the strains were prepared as described in Materials and methods. Total cellular proteins (20 µg per lane) were subjected to SDS-PAGE and immunoblotted with antibodies against Stv1p, Vph1p and Vma8p.