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Fig. 2. Effects of Cl--free artificial cerebrospinal fluid (aCSF) superfusion on integrated ({int}) whole-nerve activity recorded from isolated brainstem preparations from a bullfrog tadpole exhibiting fictive gill and lung bursts in cranial nerves VII and XII and from cranial nerve X of an adult bullfrog and an adult grass frog showing fictive lung bursts. Results are shown during superfusion with aCSF (Control), following a 20 min perfusion with Cl--free aCSF and after a 90 min washout with normal aCSF.