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Fig. 3. NOS and VIP immunoreactivities and NADPH-diaphorase reactivity in the gastrointestinal tract of Xenopus laevis. (A,B) Double-staining of the myenteric plexus (mep) of the proximal intestine showing NOS- (A) and VIP-immunoreactive nerve fibres (B) but only NOS-immunoreactive nerve cell bodies. Most VIP-immunoreactive nerve fibres are not NOS-immunoreactive. (C) NOS-immunoreactive endocrine cell in the mucosa of the proximal intestine. (D,E) Section through the proximal intestine showing colocalisation between NOS- (D) and VIP-immunoreactive nerve fibres (E) in the circular muscle layer (cm). (F,G) Double-staining of the myenteric plexus of the distal intestine showing NOS- (F) and VIP-immunoreactive nerve fibres (G) but only NOS-immunoreactive nerve cells bodies. Most VIP-immunoreactive nerve fibres are not NOS-immunoreactive. (H) NOS-immunoreactive nerve cell body in the myenteric plexus of the rectum. (I) NADPH-diaphorasereactive nerve cell body and nerve fibres in the myenteric plexus of the rectum. Bars: 25 µm (D,E,H), 50 µm (A—C,F,G,I).