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Fig. 2. PACAP, VIP and helospectin immunoreactivities in the gastrointestinal tract of Xenopus laevis. (A,B) Double-staining of a section through the cardiac stomach showing a high degree of colocalisation between PACAP (A) and VIP (B) immunoreactivities in the myenteric plexus (mep), the circular muscle layer (cm) and submucosa (sm). (C,D) A high degree of colocalisation between PACAP (C) and VIP (D) immunoreactivities in nerve fibres in the myenteric plexus of the pyloric stomach. (E,F) Colocalisation between PACAP (E) and VIP (F) immunoreactivities in varicose nerve fibres in the circular muscle layer of the proximal intestine. (G,H) Colocalisation between helospectin (G) and VIP (H) immunoreactivities in varicose nerve fibres along the circular muscle fibres in the cardiac stomach. Bars: 25 µm (E-H), 50 µm (A-D).