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Fig. 2. Plasma concentrations (mmol l-1) of Na+ ({square}), Cl- ({blacksquare}) and urea ([UNK]) during acclimation from 80% to 100% sea water in Scyliorhinus canicula. Statistical comparisons were made with time zero (*P<0.05, **P<0.01, ***P<0.001) (Student's t-test). Plasma urea concentration did not change significantly during the acclimation period. Results are expressed as means ± S.E.M. (N>=6 for each group).