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Fig. 3. The influence of 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS; 10-4 mol l-1) on intracellular pH (pHi) of gas gland cells at a constant extracellular pH (pHe) of 7.4 (A) and following an artificial intracellular acidification induced using the ammonium pulse technique (10 mmol l-1 NH4Cl in phosphate-buffered saline for 8 min) (B). {dagger}Significantly different from the control (P<0.05); *highly significantly different from the control (P<0.01). Values are means ± S.E.M.