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Fig. 5. Overall promoter structures of the Manduca sexta V-ATPase genes mvB, mvG and mvd. Hybridization screening of a genomic {lambda}-Fix II library from M. sexta led to the isolation of three genes encoding V-ATPase subunits B, G and d. The corresponding 5' upstream regions were subcloned and sequenced. Fragments of 1 kb are shown, with special emphasis on overall promoter structures in the region close to the translational start site (shaded blue). GC content was determined using a window size of 30 bp; GC contents of more than 50 % are in red, of between 20 % and 50 % are in grey and of less than 20 % are in blue. Black arrows, canonical TATA boxes; red arrows, cAMP-responsive, elements (CREs); blue arrows, ecdysterone response elements (EcREs).