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Fig. 3. Timing of the commencement of ecdysis in larvae and pupae decapitated at various times after injection of 200 pmol of ecdysis-triggering hormone (ETH). Animals were injected with ETH (at 0 min) and decapitated at the times listed on the y-axis (indicated by white arrowheads). Control larvae (C) were not decapitated. Animals decapitated 10 min after ETH injection did not ecdyse (`No ecdysis'), which is shown by a broken bar. Bars are mean times + S.E.M. Sample sizes are indicated in parentheses. Letters represent significant differences compared with controls within groups (P<0.001), as determined by analysis of variance using the Tukey test for differences ({alpha}=0.05).