Fig. 2. Photographs of single-labeled (A) and double-labeled (B-D) preparations of ganglion A3 from ecdysis-triggering hormone (ETH)-injected larvae dissected at ecdysis. Arrows in all panels point to the L_2,5 cells. (A) Video montage of 2-3 optical sections of a cGMP-stained preparation, as visualized with a color reaction. Axons of the L_2,5 cells can be seen exiting the ventral nerve (black arrowhead). (B-D) Collapsed Z-series confocal images of double-labeled preparations. Red is FMRFamide (FMRFa), leucokinin (LK) or Manduca sexta diuretic hormone (DH) staining in panels B-D, respectively. cGMP is indicated by green (B-D) and is marked by white arrows. Co-localization was not noted in cells L_2,5 in any panel. Scale bars, 100 µm.