Fig. 5. Expression of MsNSF is developmentally regulated in a subset of neuroendocrine cells and is selectively enriched in specific subsites of hormonal release in M. sexta brain. Enriched MsNSF immunoreactivity (eNSF-IR) is present on or after day 3 of pupation and continues throughout adult development (B-E). FMRFamide staining detects most cells of the neurosecretory complex and various varicosities in the brain (F). (G) Paraffin section showing exaggerated Type I staining and eNSF-IR (arrow). The image was overexposed to show both eNSF-IR and Type I staining. eNSF-IR is present only in the corpora cardiaca (CC) (H,I) and excluded from the corpora allata (CA). (I) A higher magnification of eNSF-IR in varicosities of CC in neurohemal complex. Note that no other neurosecretory cells are stained in paraffin sections (G), ruling out problems of antibody penetration in our whole mounts. A representative staining with pre-immune IgG is shown in A, for comparison of specific signals with background staining.