Fig. 1. In vivo expression of MsNSF rescues the temperature-sensitive growth defect in sec18-1 yeast mutant. (A) Wild-type (RSY 248) or strains derived from sec18-1 (SEY 5186) (Table 1) containing the `empty' vector, pYES2 (ASHY1896-1), wild-type SEC18 gene (ASHY1896-5) or SEC18 (ASHY1896-7) and MsNSF (ASHY1896-2) cloned downstream from the GAL1 promoter (P_GAL) were grown at 24 °C. Serial dilutions (indicated above panels) were spotted on to rich agar medium containing 3 % galactose and incubated at 24 °C (left), 30 °C (middle) or at 37 °C (right) for 3 days. (B) Cells were incubated at 32 °C in rich agar medium containing 3 % galactose (left), 2 % raffinose (middle) or 5 % glucose (right) as carbon source. (C) Wild-type (X2180-1B) or cells derived from sec18-1 containing various constructs were incubated at 37 °C in a humid chamber for 8 days and returned to 22 °C for 4 days.