Fig. 6. The effect of prior heat shock on time to recover (i.e. time to self-right, see text for details) of whole locusts after exposure to anoxia. Heat shock significantly altered locust sensitivity to anoxia (two-way ANOVA, F=12.82, d.f.=1, P<0.001). Values are means + S.E.M. Exposure time of locusts to anoxia had a significant effect on time to recover (two-way ANOVA, F=64.42, d.f.=5, P<0.001). There was also a significant interaction between experimental treatment (i.e. control or heat shock) and time under anoxic conditions (two-way ANOVA, F=4.58, d.f.=5, P=0.001). Compared with controls, heat-shocked locusts took longer to recover after 4 h and 5 h under anoxic conditions (two-way ANOVA with Tukey pairwise multiple-comparison, F=4.58, d.f.=5, P=0.001). All columns represent data from seven animals with the exception of heat-shock animals at 6 h, where N=5 because two animals died.