Fig. 4. The effect of prior anoxia treatment on whole-cell outward K⁺ currents of neurons in locust metathoracic ganglion slices with increasing temperature. Outward currents were obtained using voltage steps from a holding potential (V_h) of –60 mV made in 10 mV increments from –80 mV to 40 mV. (A) Representative traces of whole-cell outward K⁺ current recorded from neurons in locust metathoracic ganglion slices at different temperatures (25, 30 and 35°C) showing the current amplitude for both control and anoxia-treated animals. (B) Peak currents after anoxia treatment. When the temperature was held at 25°C, peak currents from neurons of control animals (N=9) were significantly greater than peak currents from cells of anoxia-treated animals (N=10) when V_h=–60 mV (two-way ANOVA with Tukey pairwise multiple-comparison test, F=4.58, P=0.04, d.f.=1). Anoxia treatment did not significantly alter the peak current evoked when V_h=–60 mV at 30°C (control, N=6; anoxia, N=6) or at 35°C (control, N=5; anoxia, N=6). Values are means + S.E.M. (C) Histograms of peak currents normalized to peak currents at 25°C. Control and anoxia currents were equally reduced when the temperature was increased from 25 to 30°C and to 35°C. Asterisks indicate a significant difference between control and experimental groups (P<0.05).