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Fig. 2. The effect of prior heat shock (3 h at 45°C) and anoxia (2 h in nitrogen) treatment on the survival of locusts at the normally lethal temperature of 53°C. Heat shock and anoxia pre-treatment significantly altered the thermotolerance of locusts by increasing the number of locusts alive after 0.5 h (G-test, G=10.09, d.f.=2, P<0.05) and 1 h (G-test, G=8.02, d.f.=2, P<0.05) at 53°C. Asterisks indicate a significant difference between control and experimental groups (P<0.05).