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Fig. 4. Accumulation of newly synthesized hydrocarbons (HCs) in the medium and ovary in vitro. An ovary from a day 4 female was co-incubated with abdominal sternites 3 and 4 from a day 5 female in 500 µl of TC199 medium containing 25.9 kBq of [1-14C]propionate and various concentrations of purified Blattella germanica lipophorin. (A) SDS–PAGE of hemolymph (H) and purified lipophorin (Lp). (B) Accumulation of newly synthesized hydrocarbons in the medium; r2=0.999, P<0.0001. (C) Accumulation of newly synthesized hydrocarbons in the ovary. Values are means ±1 S.E.M. (N=3–5). Some error bars are obscured by the symbol.