Fig. 2. Western blots of hsp70 isoforms, hsp90 and heat-shock factor-1 (HSF1) in a field-acclimatized (F) group of three Tegula congeners and after 30–34 days of laboratory-acclimation to 13, 18 or 23°C. Lanes contain equal amounts of protein (5 µg for hsp70 blots and 25 µg for hsp90 and HSF1 blots). A known amount of an hsp70, hsp90 or HSF1 standard (Std; see text) was loaded on each gel.