Fig. 6. The in situ expression of hsp70 genes revealed by RNA:RNA in situ hybridization using cDNA (pPW18) riboprobe (A–D and I–L) and the in situ profile of Hsp70 seen after immunostaining with the 7Fb antibody (7Fb Ab) (E–H and M–P) in the distal (A–H) and proximal (I–P) parts of adult testes (tes) and seminal vesicles (sv) in unstressed (A,E,I,M) controls (CON) or after 40 min of heat shock (B,F,J,M) and in tissues allowed to recover for 2 h (C,G,K,O) or 4 h (D,H,L,P) at room temperature (22°C) after a 40 min heat shock. The insets in A and B show the expression of hsp70 in spermatogonial cells (spg), while those in C and D show the cyst cells (cs) at higher magnification. The arrowhead in E shows the constitutive presence of Hsp70 in the spermatogonial cells. Insets in K and L show the expression of hsp70 in cyst cells (cs) in the seminal vesicles. Scale bar (applicable to all images, except to insets), 200 µm.