Fig. 5. Expression of hsp70 genes in larval testes as revealed by RNA:RNA in situ hybridization using the cDNA (pPW18) (A,E,P) 87A3'UTR (C,G,I,K,N) or 87C3'UTR (D,H,J,L,O) riboprobe and the in situ profile of Hsp70 as revealed by immunostaining with the 7Fb antibody (7Fb Ab) (B,F,M,Q) in unstressed control (CON) (A,B) or heat-shocked (HS) (C–F) testes or those allowed to recover for 30 min (G,H), 1 h (I,J), 2 h (K–M) or 4 h (N–Q) at room temperature (22°C) after a 40 min heat shock. Note the constitutive presence of Hsp70 in the anterior region (spermatogonial cells) in unstressed testes and also the greater induction of the locus 87A hsp70 genes in these cells following heat shock. Scale bar, 500 µm.