Fig. 2. Heat-shock-induced expression of hsp70 genes from the 87A and 87C loci in wild-type embryos of developmental stages 5–16 as revealed by RNA:RNA in situ hybridization (RISH) using 3'UTR riboprobes of hsp70 genes from the 87A (A,C,E,G,I,K,N,Q,T,W) or the 87C (B,D,F,H,J,L,O,R,U,X) locus. Stages of the embryos are indicated on the right (D, L and V in this column refer to the dorsal, lateral or ventral views, respectively, of the embryo). The arrow in A points to the pole cells. The black arrowheads in E and F and the small white arrowheads in F, G and H indicate the neuroblasts in the procephalic region and ventral nerve cord, respectively. The white arrows in I and J indicate the proneural clusters in stage 11 embryos. The white arrowheads in K, R and U refer to the glial cells. The small black arrows in N, O and P point to the tracheal pits, while the long black arrows in Q, R, S and T show the peripheral nervous system cells. Insets in T, U and V show the clusters of neuroblasts (T and V) and glial cells (U) from the respective embryos. Embryos in M, P, S and V are immunostained with the BP104 antibody to show the embryonic central and peripheral nervous systems for comparison with RISH patterns in K–U. Scale bar (applicable to all parts of the figure, except the insets in T, U and V, which are at higher magnification), 200 µm.