Fig. 8. Induction of vitellogenin (Vtg) mRNA in eelpout embryos exposed to 4-tert-octylphenol (4-tOP) in ovario for 35 days. Ethidium-bromide-stained agarose gel showing the amplified RT-PCR products using Vtg-(215 bp) or ß-actin- (340 bp) specific primers on total RNA extracted from embryos dissected out of mother fish exposed to isopropanol (control), 25 µgl^-1 4-tOP (OP25), 100 µgl^-1 4-tOP (OP100) or 0.5 µgl^-1 17ß-oestradiol (E₂). Three embryos were pooled per mother fish. Numbers indicate different mother fish. M, 100 bp marker; Neg, negative control (no RT-enzyme).