Fig. 6. Effect of SB203580 on 2-deoxyglucose (2-DOG) uptake and p38 activation. C2C12 cells, differentiated in the absence (MF) or in the chronic presence (MFI) of insulin, were incubated in Krebs-Ringer phosphate (KRP) buffer for 30 min followed by another incubation of 30 min in the presence of 10 µmol l^-1 SB203580. Cells were stimulated with 100 nmol l^-1 insulin in the presence of SB203580, followed by 2-DOG uptake for 10 min (A) or immunoblot analysis of p38 activation (B). Cells were treated with 2 mmol l^-1 gliclazide during the last 24 h of differentiation. Error bars represent the S.E.M. of three independent experiments.