Fig. 4. Effect of the chronic presence of insulin and gliclazide on 2-deoxyglucose (2-DOG) uptake in C2C12 myotubes. C2C12 cells, differentiated in the absence (MF) or in the chronic presence (MFI) of insulin, were washed with Krebs-Ringer phosphate (KRP) buffer as described in Materials and methods, followed by stimulation with insulin (100 nmol l^-1) for 15 min. [³H]2-DOG uptake was measured as described in Materials and methods. Cells were treated with 2 mmol l^-1 gliclazide during the last 24 h of differentiation. Error bars represent the S.E.M. of four independent experiments (^*P<0.05).