JEB -- Atia et al. 205 (23): 3729 Figure IG3

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Fig. 3. Effects of the Nigella arvensis (NA) extract on kinetic parameters k_ob, k_bo and K_B of the interaction of 6-chloro-3,5-diaminopyrazine-2-carboxamide (CDPC) with the Na⁺ channel. (A) Effect of stepwise application of apical CDPC concentrations (ranging from 10 µmol l^-1 to 100 µmol l^-1) on short-circuit current (I_sc) and transepithelial conductance (G_T) of A6 cells before and after basolateral (Bl) stimulation with 250 µl l^-1 NA extract (N=6). 50 µmol l^-1 amiloride (Ami) was added after the highest dose of CDPC at the end of the experiment. Solutions were as in Fig. 1. (B) 2 ${pi}$ f_c data at different doses of apical CDPC ([CDPC]_Ap). The slope and intercept of the linear regressions for the control (2 ${pi}$ f_c=7.66[CDPC]_Ap+259.29) and in the presence of NA extract (2 ${pi}$ f_c=7.64 [CDPC]_Ap+281.51) indicate the ON and OFF rates, respectively, of the interaction between CDPC and the Na⁺ channel.