Fig. 2. Maternal lipid localization in neonate mosquito larvae. Purified lipophorin was double labeled with Texas Red-PE and BODIPY-FA before injecting into vitellogenic females; eggs were collected and kept in water at 28°C until larval hatching. The fluorescence associated with newborn larvae was examined within 30 min after hatching. (A) Localization of BODIPY-FA in A. gambiae larvae. (B) Localization of Texas Red-PE in the same newborn larvae as shown in A. (C) Merged image of A and B shows differential localization of BODIPY-FA and Texas Red-PE. (D) Phase-contrast image of the same larvae as shown in C. (E) All neonate larvae hatched from the same batch of eggs, showing identical pattern of differential partitioning of fatty acid (BODIPY-FA) and phosphatidylethanolamine (Texas Red-PE). (F) Phase-contrast image of the larvae shown in E. (G) Maternal lipid distribution in the larvae of A. aegypti mosquitoes. (H) Phase-contrast image of larvae shown in G. Scale bar, 200 µm.