Fig. 8. Natural and peptide-induced eclosion behavioural sequences of pharate adults. (A) Natural behaviour was initiated by abdominal rotations every 2-5 s for approximately 40 min, followed by a quiescent phase for approximately 50 min and eclosion peristaltic movements for 12-15 min. Injection of pre-ecdysis-triggering hormone (PETH) or ecdysis-triggering hormone (ETH) induced the entire behavioural sequence, but the onset of ecdysis was accelerated in ETH-treated animals. (B) Separate injections of each peptide caused 40-80 rotations of the abdomen of pharate adults, each lasting 1-2 s with intervals of 2-6 s. (C) After a quiescent phase, pharate adults initiated peristaltic movements of the abdominal segments (arrowhead).