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Fig. 2. Pre-ecdysis-triggering hormone (PETH)- and ecdysis-triggering hormone (ETH)-immunoreactivity in Inka cells before and after pupal ecdysis and adult eclosion. Intense PETH-immunoreactivity in Inka cells of pharate pupa (A) was reduced 5 min after ecdysis (B, arrow). Strong staining with ETH antiserum in Inka cells of pharate adult (C) decreased considerably 5 min after eclosion (D, arrow). Scale bar, 200 µm.