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Fig. 10. Ecdysis-triggering hormone (ETH)-induced pre-ecdysis and ecdysis bursts in the isolated central nervous system (CNS) of pharate pupae in vitro. (A) Asynchronous pre-ecdysis I bursts in dorsal nerves of AG5-8D of an isolated chain of abdominal ganglia (AG1-8). (B) Approximately 40 min later, this isolated chain of AG1-8 switched to ecdysis bursts. The ecdysis motor pattern recorded from isolated AG1-8 closely resembled that observed in the intact CNS. Calibration bars: horizontal, 5 s; vertical, 10 µV.