Fig. 3. (A) The contractile vacuole (CV) potential (filled squares) and the rate of fluid segregation in the CV complex (R_CVC; open circles) as a function of the osmolarity of adaptation solution in Paramecium multimicronucleatum. Values are means ± S.E.M. (N=4-10). (B) DS-1 labelling of the decorated spongiome, visualized by its immunological fluorescence image. The number at the top of each picture corresponds to the osmolarity (mosmoll^-1) to which the cell was adapted. Scale bar, 20 µm.