Fig. 3. Production of all detected cuticular hydrocarbons in 4- day-old flies after a single heat shock induced in adults 9 h old. Values are means of absolute amounts (ng) ± S.E.M. for heat-shocked (HS 9h; black bars) and non-heat-shocked (no HS; white bars) males (A—D) and females (E—F) of the hsp70-GAL4 x UAS-tra (hsp-tra) genotype. For genotypes, see Fig. 1. Hydrocarbon nomenclature: 9-T, cis 9-tricosene; 7-T, cis7-tricosene; 5-T, cis 5-tricosene; 23LIN, n-tricosane; 7,11PD, cis,cis7,11-pentacosadiene; 9-P, cis 9-pentacosene; 7-P, cis 7-pentacosene; 25LIN, n-pentacosane; 7,11HD, cis,cis7,11-heptacosadiene; 27BR, 2-methylhexacosane; 7-H, cis7-heptacosene; 27LIN, n-heptacosane; 7,11ND, cis,cis7,11-nonacosadiene; 29BR, 2-methyl-octacosane. The total of CHs shown here represents 88% of all detected CHs. For each genotype, N=10-20.