Fig. 4. Electrophoretic mobility-shift assay (EMSA) of HSF1 activation in liver tissue from G. mirabilis. Representative activation profiles of HSF1 in the liver tissues of differently acclimated G. mirabilis in response to heat shock (mean HSF1 activation levels for N=5 samples from each of the groups shown in Fig. 5). EMSAs were run as described in the legend to Fig. 3 and Materials and methods. Heat shock temperatures are labeled below each lane and acclimation groups are shown to the right of the figure.