Fig. 2. Representative protein synthesis patterns in response to heat shock in the liver tissue of Gillichthys mirabilis (N=3) acclimated to (A) 21°C and (B) 28°C. Proteins were radiolabelled with ³⁵S-methionine/cysteine after heat shock for 1 h at the temperatures listed below each lane. After heat shock, proteins were separated on 7.5% polyacrylamide gels, which were dried and exposed to X-ray films. The positions of molecular mass markers are denoted on right (arrows). N=3 for both acclimation groups.