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Fig. 7. Effect of NO donors and NOS inhibitors on the amplitude of rotational surges. Both 10 µmol l-1 SNAP (A) and 25 µm SNP (B) caused a significant increase in the mean amplitude of rotational surges, expressed in rotations per minute (r.p.m.). In contrast, 10 mmol l-1 L-NAME (C) and 100 µmol1-1 7-NI (D) had no effect on the mean amplitude of rotational surges. Asterisks represent statistically significant differences compared to the control (artificial pondwater, APW) treatment. (A) P<0.05, N=44 surges from 34 embryos; (B) P<0.05, N=39 surges from 30 embryos; (C) N=15 surges from 17 embryos; (D) N=13 surges from 23 embryos.