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Fig. 6. Effect of NO donors and NOS inhibitors on the frequency of rotational surges. Both 10 µmol l-1 SNAP (A) and 25 µmol l-1 SNP (B) had no effect on the frequency of rotational surges. In contrast, 10 mmol l-1 L-NAME (C) and 100 µmol l-1 7-NI (D) caused a significant reduction in the frequency of rotational surges. Asterisks represent statistically significant differences (P<0.001) compared to the control (artificial pondwater, APW) treatment. (A) N=34 embryos; (B) N=30 embryos; (C) N=17 embryos; (D) N=23 embryos.